FIG. 2.
Maintenance of HSV-specific, memory CD4 T cells. Groups of BALB/c mice were immunized with 2 × 105 PFU of 5BlacZ, 5B86, or an equivalent amount of control supernatant. After 1 month, splenocytes were cultured with or without HSV-2 gD245-259 peptide or UV-inactivated HSV-2 (UV HSV-2) for 20 h on plates coated with anti-IFN-γ antibody. Cytokine-secreting cells (CSC) were then enumerated by an ELISPOT assay. Data represent the mean ± the standard error of the mean (SEM) of triplicate wells. The experiment was repeated once. **, P value of 0.0024; ***, P value of 0.0002 for 5B86 compared with 5BlacZ.