FIG. 4.

Titers of challenge virus shed from the genital mucosa. Groups of BALB/c mice were immunized with high (A), medium (B), or low (C) doses of supernatant-derived 5BlacZ or 5B86. Data for a group of mice immunized with control supernatant (control supe) in an amount equivalent to the high dose of virus are shown on all three graphs for comparison. All mice were challenged 1 month after immunization by i.vag. infection with HSV-2 strain G-6. Titers of virus collected by vaginal swab at the indicated times postinfection were determined by standard plaque assay. Data represent the geometric mean ± SEM for 10 samples compiled from two independent experiments. *, P value between 0.0495 and 0.0381; **, P value of 0.0022; ***, P value between 0.0013 and 0.0005 for 5B86 compared with 5BlacZ.