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Cosmid clones used to recover recombinant viruses. (A) Organization of the serotype 1 MDV genome. (B) Schematic representation of the overlapping cosmid clones used to reconstitute recombinant viruses rMd5 and rMeqGCN, derived from a very virulent strain of MDV (Md5). (C) Location of EcoQ fragment and Meq gene in cosmids SN5 and A6. (D) Location of KpnI site described in Materials and Methods, located within the EcoQ fragment at nucleotide 385 of the meq gene.
