FIG. 4.

EMSAs of Meq and MeqGCN protein were performed to test the DNA binding capacity of MeqGCN. Purified baculovirus-expressed proteins, Meq and MeqGCN, were incubated with radiolabeled oligonucleotide probes, followed by gel retardation analysis. Two different probes were used: a consensus AP-1 oligonucleotide and an ACACA probe derived from the MDV origin of replication (MDV Ori). Band shifts are observed by Meq and MeqGCN (black arrow), the intensity of which decreases in the presence of unlabeled competitor. NS, nonspecific bands.