Growth of VSV/FAST in vitro. Confluent monolayers of Vero cells were infected in triplicate with either VSV/GFP or VSV/FAST at a multiplicity of infection of 5.0 for 45 min in minimal medium, after which the monolayers were washed three times. Samples were collected at 0, 4, 8, 12, and 24 h postinfection, and virus titers were determined in duplicate by plaque assay on Vero cells using standard techniques. The mean of the log-transformed titers are shown ± the standard error of the mean (SEM).