FIG. 3.

Assessment of viral protein expression. (A) HeLa cells were mock transfected (control) or transfected with the WT or mutant proviruses, and equal volumes of cell and virion lysates were subjected to SDS-PAGE followed by Western blotting analysis using the 902, Chessie 8, and 183 MAbs. (B) Cell-free culture supernatants obtained from acutely infected CEM-SS cells at the peak of viral replication were concentrated by ultracentrifugation and reconstituted with RPMI containing 1% FBS, and p24 levels were determined. WT and mutant virions containing equal amounts of p24 were resolved by SDS-PAGE followed by Western blotting using the 902, Chessie 8, and 183 MAbs.