TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′[traD36 proAB+lacIqlacZ ΔM15] | TakaraBio |
| JE5513Tc | Hfr man1 pps lpp2 thyA uvrC279::Tn10 | 3 |
| YA5513Tc | JE5513Tc pgsA3 | 3 |
| S. aureus | ||
| RN4220 | Restriction minus wild type | 37 |
| TS2519 | RN4220 ltaS2519 | This work |
| TS2947 | RN4220 ltaS2947 | This work |
| TS8643 | RN4220 ltaS8643 | This work |
| TS2519kan | TS2519 Ω(SA0667-SA0668::pSF151kan) | This work |
| TS2947kan | TS2947 Ω(SA0667-SA0668::pSF151kan) | This work |
| TS8643kan | TS8643 Ω(SA0667-SA0668::pSF151kan) | This work |
| M0674/pM101 | RN4200 ΔltaS::phleo/pM101 | This work |
| M0674/pM101-ltaS | RN4200 ΔltaS::phleo/pM101-ltaS | This work |
| M0674/pM102-ltaS | RN4200 ΔltaS::phleo/pM102-ltaS | This work |
| M0107 | RN4220 Δspa::phleo | This work |
| M0702 | RN4220 ΔtagO::pT0702 | 24 |
| M0875 | RN4220 ΔypfP::pT0875 | 24 |
| YO1001/pM101 | M0674 ΔypfP::pT0875/pM101 | This work |
| YO1001/pM102-ltaS | M0674 ΔypfP::pT0875/pM102-ltaS | This work |
| YO1003/pM102-ltaS | M0674 ΔtagO::pT0702/pM102-ltaS | This work |
| Plasmids | ||
| pND50 | S. aureus-E. coli shuttle vector; Cmr | 54 |
| pKE515 | S. aureus-E. coli shuttle vector, Cmr Ampr | 30 |
| pSltaS | pKE515 carrying the ltaS gene | This work |
| pSF151 | E. coli pUC plasmid; Kmr | 47 |
| pSF-ltaS | pSF151 carrying ΔltaS::phleo | This work |
| pM101 | S. aureus-E. coli shuttle vector; Kmr | Matsuo et al., unpublished |
| pM102 | S. aureus-E. coli shuttle vector; Cmr | Matsuo et al., unpublished |
| pM101-ltaS | pM101 carrying the ltaS gene | This work |
| pM102-ltaS | pM102 carrying the ltaS gene | This work |
| pHW353 | S. aureus-E. coli shuttle vector carrying TetR and xyl-tet | 13 |
| pNDX1 | pND50-based S. aureus-E. coli shuttle vector carrying TetR and xyl/tet from pWH353 | This work |
| pNDXltaSHis | pNDX1 carrying His-tagged LtaS ORF | This work |
| pBAD24 | Inducible expression vector in E. coli | 17 |
| pBADltaSHis | pBAD24 carrying His-tagged LtaS ORF | This work |