TABLE 2.
Complementation of temperature-sensitive mutant strainsa
| Strain | No. of transformants (103) with the following plasmid at 43°C/30°C:
|
|
|---|---|---|
| pSltaS | pKE515 | |
| TS2519 | 2.1/1.3 | 0.0/0.8 |
| TS2947 | 1.0/2.0 | 0.0/2.6 |
| TS8643 | 5.0/4.4 | 0.0/1.6 |
a
Competent cells of each temperature-sensitive mutant strain of S. aureus (50 μl) were electroporated with 250 ng of pSltaS or the empty vector pKE515, followed by incubation at 43°C or 30°C for 24 h on LB agar plates containing 12.5 μg/ml Cm. The number of transformants was counted. Data are representative of more than three independent experiments.