TABLE 5.
Deficiency of both LTA and WTA leads to synthetic lethality
| Temp (°C) | No. of transformantsa on the following medium with the indicated plasmid:
|
|||
|---|---|---|---|---|
| LB
|
LB + sucrose
|
|||
| pM101 | pM101-ltaS | pM101 | pM101-ltaS | |
| 30 | 20b | 1,581 | 0 | 868 |
| 37 | 4b | 1,670 | 0 | 929 |
| 43 | 0 | 1,250 | 0 | 920 |
a
YO1003 (ΔltaS ΔtagO) harboring pM102-ltaS was transformed with pM101 or pM101-ltaS and selected with Km on LB agar plates with or without 40% sucrose for 48 h. Data are representative of at least three independent experiments.
b
Tiny colonies appeared but failed to regrow to detectable levels under the same agar plate conditions after being picked from the original agar plates.