TABLE 2.
Comparison of relative protein spot intensities after 1O2 exposure
| Category and locus tag | Protein | Function | Relative protein spot intensity (%) ± SDa
|
||
|---|---|---|---|---|---|
| 2.4.1ΔrpoHII | 2.4.1ΔrpoHII (pRK2.4.1rpoHII) | 2.4.1ΔrpoHII (pRK2.4.1rpoHIIRSP0600) | |||
| Category 1 | |||||
| RSP0392† | GloA | Predicted lactoylglutathione lyase: glyoxalase I | 8 ± 4 | 116 ± 12 | 98 ± 11 |
| RSP3272† | Ggt | γ-Glutamyltranspeptidase | 0.0 ± 0.0 | 38 ± 5 | 29 ± 9 |
| RSP3537† | Adh | Zn-containing alcohol dehydrogenase | 0.0 ± 0.0 | 169 ± 25 | 148 ± 2 |
| RSP2314† | Predicted oxidoreductase | 11 ± 5 | 63 ± 16 | 105 ± 7 | |
| RSP3075† | Hypothetical protein | 9 ± 1 | 74 ± 0.7 | 95 ± 2 | |
| RSP3076 | Hypothetical protein | 0.0 ± 1 | 161 ± 11 | 152 ± 9 | |
| RSP1591 | Gst | Predicted glutathioneS-transferase | 0.0 ± 0.0 | 93 ± 2 | 30 ± 10 |
| RSP2645 | Eda | KDPG/KHG bifunctional aldolase | 39 ± 23 | 97 ± 6 | 107 ± 16 |
| RSP2973 | PrxQ | Peroxiredoxin | 20 ± 9 | 159 ± 37 | 91 ± 23 |
| RSP2860 | RpsB | 30S ribosomal protein S2 | 192 ± 44 | 93 ± 9 | 110 ± 5 |
| RSP0423 | Predicted oxidoreductases related to aryl-alcohol dehydrogenases | 30 ± 3 | 111 ± 33 | 90 ± 40 | |
| RSP2268 | Predicted metalloβ-lactamase | 0.0 ± 0.0 | 113 ± 6 | 109 ± 11 | |
| RSP1852 | Hypothetical protein | 147 ± 40 | 108 ± 14 | 117 ± 1 | |
| RSP1464 | DsbG | Putative periplasmic thiol-disulfide interchange protein of the DsbA family | 82 ± 26 | 94 ± 24 | 81 ± 11 |
| Category 2 | |||||
| RSP3164† | Ferredoxin-like protein | 0.0 ± 0.0 | 74 ± 10 | 73 ± 21 | |
| RSP3342 | Bfr | Bacterioferritin | 0.0 ± 0.0 | 147 ± 31 | 160 ± 15 |
| RSP2481 | CysE | Serine acetyltransferase | 35 ± 13 | 62 ± 6 | 59 ± 18 |
| RSP0799 | GloB | Putative hydroxyacylglutathione hydrolase: glyoxalase II | 7 ± 4 | 63 ± 3 | 58 ± 6 |
| RSP2294 | GloB | Putative hydroxyacylglutathione hydrolase: glyoxalase II | 2 ± 1 | 95 ± 1 | 87 ± 11 |
| RSP1397 | Gst | GlutathioneS-transferase | 15 ± 13 | 106 ± 49 | 112 ± 5 |
| RSP2735 | Pgl | Inner membrane subunit of ABC sugar transporter | 32 ± 15 | 112 ± 13 | 104 ± 4 |
| RSP1096 | PqqL | Putative zinc peptidase | 7 ± 5 | 76 ± 8 | 51 ± 0.8 |
| RSP1097 | PqqL | Putative zinc peptidase | 42 ± 12 | 47 ± 19 | 39 ± 0.5 |
| RSP0663 | Formate-tetrahydrofolate ligase | 6 ± 4 | 81 ± 54 | 51 ± 41 | |
| RSP1415 | Putative polysaccharide deacetylase | 0.4 ± 0.0 | 78 ± 0.3 | 79 ± 4 | |
| RSP1507 | NADH-dependent aldehyde dehydrogenase | 4 ± 1 | 35 ± 15 | 49 ± 9 | |
| RSP2779 | CatA | Catalase | 37 ± 17 | 129 ± 55 | 117 ± 49 |
| RSP0355 | Putative serine protease involved in heat shock response, exhibiting chaperone function | 96 ± 25 | 75 ± 36 | 65 ± 3 | |
| RSP0558 | Possible ribosomal protein L11: predicted methyltransferase | 83 ± 43 | 70 ± 0.4 | 65 ± 0.6 | |
| RSP0967 | SucC | Succinyl-coenzyme A synthetase; β subunit | 120 ± 53 | 98 ± 5 | 109 ± 6 |
| RSP0966 | SucD | Succinyl-coenzyme A synthetase; α subunit | 127 ± 59 | 90 ± 9 | 88 ± 25 |
| RSP1410 | Hypothetical protein | 49 ± 46 | 48 ± 3 | 49 ± 2 | |
| RSP2241 | HisH | Phosphoribosyl-ATP pyrophosphatase | 133 ± 58 | 100 ± 0.1 | 100 ± 0.1 |
| Category 3 | |||||
| RSP0847 | OmpR | Two-component transcriptional regulator | 117 ± 66 | 131 ± 32 | 126 ± 3 |
a
Protein spot intensities ± standard deviations (SD) were normalized to values observed for the wild-type strain 2.4.1 and are shown for the rpoHII mutant strain 2.4.1ΔrpoHII and for the complemented mutants harboring the plasmid pRK2.4.1rpoHII or pRK2.4.1rpoHIIRSP0600. Category 1 includes RpoE-dependent proteins, category 2 contains conditionally RpoE-dependent proteins, and category 3 contains RpoE-independent proteins (17). Boldface proteins depend on RpoHII; †, predicted RpoHII target sequence (6). Mean values of three independent experiments are depicted for the rpoHII mutant and for the two complemented mutants.