TABLE 2.
Amino acid-rich minimal mediuma
| Medium component | Concn (mM) in modified amino acid-rich medium |
|---|---|
| Ala | 2.9 |
| Arg | 2.35 |
| Asn | 0 |
| Asp | 3.25 |
| Cys | 0.2 |
| Gln | 0 |
| Glu | 12.4 |
| Gly | 2.4 |
| H2O | —b |
| Heme | 10 |
| His | 1.35 |
| Ile | 2.2 |
| Leu | 4.65 |
| Lys | 3.15 |
| Met | 0.55 |
| Phe | 1.9 |
| Phosphate | 10 |
| Pimelate | 10 |
| Pro | 5.55 |
| Ser | 4.05 |
| Thiamine | 10 |
| Thr | 2.75 |
| Trp | 1 |
| Tyr | 0.9 |
| Val | 3.9 |
a
Modified from Takahashi et al. (90). Pimelate and thiamine are not present in the original media but are required for the current model. Pimelate, thiamine, phosphate, and heme are added in excess to make sure that they are not limiting factors.
b
—, Water uptake is not constrained in the model.