TABLE 1.
S. coelicolor A3(2) strains used in this study
| Strain | Genotypea | Isolationb | Reference or source |
|---|---|---|---|
| 2709 | proA1 hisA1 argA1 cysD18 uraA1 strA1 SCP1− SCP2− | 27 | |
| HJ2 | Δsmc::hyg | M145 × pHJ2 | This study |
| J2537 | ΔparB::acc(3)IV | 28 | |
| J2540 | ΔparB::acc(3)IV/parB+ | J2537::pIJ6539 | 28 |
| JM148 | ΔftsK::aphI (aphI inserted in same orientation as ftsK′) | M145 × pJM148 | This study |
| JM152 | ΔftsK::aphI (aphI inserted in opposite orientation from ftsK′) | M145 × pJM152 | This study |
| M145 | Prototroph SCP1− SCP2− | 27 | |
| RMD1 | ftsK-egfp acc(3)IV | M145 × 7C7ftsK-egfp | This study |
| RMD2 | smc-egfp acc(3)IV | M145 × 7A1smc-egfp | This study |
| RMD3 | Δsmc::hyg ΔftsK::aphI | HJ2 × JM148 | This study |
| RMD4 | Δsmc::hyg ΔparB::acc(3)IV | HJ2 × J2537 | This study |
| RMD5 | ΔparB::acc(3)IV ΔftsK::aphI | J2537 × JM148 | This study |
| RMD6 | Δsmc::hyg ΔparB::acc(3)IV ΔftsK::aphI | RMD3 × J2537 | This study |
| RMD7 | Δsmc::hyg/smc+ | HJ2/pHW35 | This study |
| RMD8 | ΔftsK::aphI/ftsK+ | JM148/pRMD6 | This study |
| RMD9 | Δsmc::hyg ΔftsK::aphI/ftsK+ | RMD3/pRMD6 | This study |
| RMD10 | Δsmc::hyg ΔftsK::aphI/smc+ | RMD3/pHW35 | This study |
| RMD11 | Δsmc::hyg ΔparB::acc(3)IV/smc+ | RMD4/pHW35 | This study |
| RMD12 | Δsmc::hyg ΔparB::acc(3)IV/parB+ | J2540 × HJ2 | This study |
| RMD13 | ΔparB::acc(3)IV ΔftsK::aphI/ftsK+ | RMD5/pRMD6 | This study |
| RMD14 | ΔparB::acc(3)IV ΔftsK::aphI/parB+ | J2540 × RMD5 | This study |
| RMD15 | Δsmc::hyg ΔparB::acc(3)IV ΔftsK::aphI/ftsK+ | RMD6/pRMD6 | This study |
| RMD16 | Δsmc::hyg ΔparB::acc(3)IV ΔftsK::aphI/smc+ | RMD6/pHW35 | This study |
| RMD17 | ΔSCO1768::acc(3)IV | M145 × pRMD7 | This study |
| RMD18 | ΔscpA::acc(3)IV | M145 × pRMD8 | This study |
| RMD19 | ΔscpAB::acc(3)IV | M145 × pRMD9 | This study |
| RMD20 | Δsmc::hyg ΔscpA::acc(3)IV | HJ2 × RMD18 | This study |
| RMD21 | Δsmc::hyg ΔscpAB::acc(3)IV | HJ2 × RMD19 | This study |
| RMD22 | ΔscpA::acc(3)IV/scpAB+ | RMD18/pRMD10 | This study |
| RMD23 | ΔscpAB::acc(3)IV/scpAB+ | RMD19/pRMD10 | This study |
| RMD24 | ΔftsK::acc(3)IV (null, deletion begins upstream of 5′ end) | M145 × pAEB232 | This study |
a
All mutants are derivatives of prototrophic strain M145.
b
Strains were constructed by protoplast transformation: the recipient strain is listed first and the source of donor DNA second (e.g., HJ2 × JM148 indicates that HJ2 was transformed with DNA prepared from JM148), or the recipient strain is listed first and the mutagenic plasmid second (e.g., M145 × pHJ2).