TABLE 2.
Plasmids and cosmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| SC7A1 | Cosmid source of smc | 43 |
| SC7C7 | Cosmid source of ftsK | 43 |
| SCI51 | Cosmid source of scpAB | 43 |
| H24parB-egfp | egfp inserted in frame 3′ of parB in cosmid SCH24; acc(3)IV aphII | 25 |
| 7A1smc-egfp | egfp inserted in frame 3′ of smc in cosmid SC7A1; acc(3)IV aphII | This study |
| 7C7ftsK-egfp | egfp inserted in frame 3′ of ftsK in cosmid SC7C7; acc(3)IV aphII | This study |
| pAEB228 | 4.2-kb PvuII fragment from SC7C7 containing ftsK cloned into EcoRV-digested pBluescript (ftsK; same orientation as lacZ′) | This study |
| pAEB232 | 2.5-kb MluI-SphI deletion of pJR143 [ΔftsK::acc(3)IV; same orientation as ftsK]; aadA | This study |
| pAH137 | Source of neomycin resistance gene aphI | A. Hausler, unpublished data |
| pBluescriptII SK(+) | Standard cloning vector | Stratagene |
| pIJ773 | Source of acc(3)IV for in vivo recombination | 19 |
| pIJ6539 | 5.3-kb EcoRI fragment containing parB; tsr (parB complementation plasmid) | 28 |
| pKC1053 | Source of hygromycin resistance gene hyg | 29 |
| pHJ2 | 3.2-kb SalI-PstI deletion of pHW25 (Δsmc::hyg; same orientation as smc); acc(3)IV | This study |
| pHW24 | 5.4-kb XhoI fragment from SC7A1 cloned into pBluescript (smc; opposite orientation from lacZ′) | This study |
| pHW25 | 5.4-kb XhoI fragment from SC7A1 cloned into pBluescript (smc; same orientation as lacZ′) | This study |
| pHW35 | 24-kb BamHI-EcoRI fragment from pJRM10 cloned into pHW24 (smc complementation plasmid) | This study |
| pJR143 | 6.1-kb BamHI-PstI fragment of SC7C7 containing ftsK cloned into pOJ260 (ftsK; same orientation as lacZ′); acc(3)IV | This study |
| pJR148 | 1.8-kb SphI fragment deletion of pJR143 (ΔftsK::aphI; same orientation as ftsK′); acc(3)IV | This study |
| pJR152 | 1.8-kb SphI fragment deletion of pJR143 (ΔftsK::aphI; opposite orientation from ftsK′); acc(3)IV | This study |
| pJRM10 | Bifunctional cloning vector, low copy number in S. coelicolor; bla tsr | 35 |
| pOJ260 | pUC-like plasmid containing oriT and acc(3)IV in place of bla | 4 |
| pOJ427 | Source of apramycin resistance gene acc(3)IV for in vitro recombination | B. Schoner, unpublished data |
| pRMD6 | 24-kb EcoRI-HindIII fragment from pJRM10 cloned into pAEB228 (ftsK complementation plasmid) | This study |
| pRMD7 | Cosmid SCI51 containing ΔSCO1768::acc(3)IV by in vivo recombination; aphII | This study |
| pRMD8 | Cosmid SCI51 containing ΔscpA::acc(3)IV by in vivo recombination; aphII | This study |
| pRMD9 | Cosmid SCI51 containing ΔscpAB::acc(3)IV by in vivo recombination; aphII | This study |
| pRMD10 | 4.8-kb XbaI fragment from SCI51 containing scpAB cloned into pBluescript (scpAB; opposite orientation from lacZ′) | This study |
| pRMD11 | 24-kb HindIII-SpeI fragment from pJRM10 cloned into pRMD10 (scpAB complementation plasmid) | This study |