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. 2008 Oct 17;191(1):115–122. doi: 10.1128/JB.00841-08

FIG. 2.

FIG. 2.

Mapping the promoter elements of hmuY of P. gingivalis 33277. (A) cDNA of hmuY mRNA was generated by 5′ RACE-PCR. The RACE-PCR products were visualized on a 1.5% agarose gel. Lane 1, molecular size standards; lane 2, outer 5′ RACE-PCR product; lane 3, inner 5′ RACE-PCR product. (B) DNA sequence of the hmuY promoter region. The transcriptional start site A (+1) established by 5′ RACE-PCR and the potential start codon, ATG, are bolded. The primers used for RACE -PCR are underlined. The potential Fur box, −10, and −35 regions are shaded. (C) RT-PCR analysis of hmuY mRNA. Lane 1, 100-bp ladder marker. Lanes 2, 3, and 4 are RT-PCR products of 33277. Lane 2, RT-PCR with primers RTF1 (from −55 to −36) and RTR4 (from +347 to +366); lane 3, RT-PCR undertaken with primers RTF2 (from +4 to +24) and RTR4 (from +347 to +366); lane 4, RT-PCR RTF3 (from +215 to +234) and RTR4 (from +347 to +366). Lanes 5 and 6 are RT-PCR products of W83. Lane 5, RT-PCR with primers RTF1 (from −55 to −36) and RTR4 (from +347 to +366); lane 6, RT-PCR RTF3 (from +215 to +234) and RTR4 (from +347 to +366).