TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| P. gingivalis | ||
| 33277 | Type strain from ATCC | Lab collection |
| 1237E | P. gingivalis mutant with the pg1237 gene inactivated by insertion of an ermF-ermAM cassette; Emr | This study |
| Plasmids | ||
| PCRII-TOPO | Linearized plasmid with single 3′ deoxyribosylthymine residues; Kmr Amr | Invitrogen |
| pTOPO1237 | PCRII-TOPO plasmid carrying a pg1237 gene | This study |
| pET30b | Circle plasmid carrying an N-terminal His tag/thrombin/enterokinase configuration plus an optional C-terminal His tag sequence | Novagen |
| pET30b-1237 | pET30b plasmid carrying a pg1237 gene in the expression region | This study |
a
Kmr, Emr, and Amr indicate resistance to kanamycin, erythromycin, and ampicillin, respectively.