TABLE 2.
Oligonucleotide primers
| Gene | Primer | Primer sequence (5′-3′)a | Application |
|---|---|---|---|
| pg1237 | r1237F-EcoRV | CAGTCGATATCCATGAATATCATCACACTCAAGG | Full-length pg1237 open reading frame amplification |
| r1237R-HindIII | CAGTCAAGCTTTTAGTGGGCGATTTCGTCGA | ||
| pg1237(1)F | GCATACGAGAAGTCTGTGAGGA | Construction of pg1237 mutation | |
| pg1237(1)R-erm | GATGTTGCAAATACCGATGAGCAATCGGCGTAGTGAGCAAAC | ||
| pg1237(2)F-erm | CCTCTAGAGTCGACCTGCAGCGAAGAGAGCGGAGAAGAAC | ||
| pg1237(2)R | GAAACGGTCAAGCAGTGGAT | ||
| pg1237-164F | CCACGCCACAGTAGAGGAAT | Real-time PCR | |
| pg1237-164R | GCTCTTCGGCAATCTCTTTG | ||
| ermF-ermAM | ErmF | GCTCATCGGTATTTGCAACA | ermF-ermAM amplification |
| ErmR | CTGCAGGTCGACTCTAGAGG | ||
| pg1551 (hmuY) | pg1551-149F | GGCTACTACCGTTCCGACAG | Real-time PCR |
| pg1551-149R | ATCCCTGTGCGTTCTTCTTG | ||
| TSR1 | TTCGGGTGTGGAGGGTTGGT | Identification of transcriptional start site | |
| TSR2 | CGGCTCGTCTTTCTTCTTCC | ||
| RTR4 | CTGTCGGAACGGTAGTAGCC | ||
| RTF1 | CCTACAAATTGGGATTGCTC | ||
| RTF2 | CCTATTAAAAGTAAGGTCAG | ||
| RTF3 | AAATGTTACCGACTATAAGA | ||
| Prom-F | Bio-ACCTATGTATCGAGGGCTTT | Generating biotin-labeled hmuY promoter region for EMSA | |
| Prom-R1 | CTGACCTTACTTTTAATAGG | ||
| Prom-R2 | CACAGAGTGCGGAGAAAATG | ||
| pg1552 (hmuR) | pg1552-134F | GCGACGGACAGAAATACGAT | Real-time PCR |
| pg1552-134R | GCCTGCAACATTCAGTTCCT | ||
| pg1737 | pg1737-131F | ATGAATCCGATCCGCCACCAC | Real-time PCR |
| pg1737-131R | GCCTCCCATCCCAAAGCACT | ||
| tlr | 5′tlr-454 | CCTGCGGGAACGGACAATATC | Real-time PCR |
| 3′tlr-630 | GCTACCGCCGAAGAGAGAAAC | ||
| fetB (ihtB) | 5′ihtB-636 | TATTGCCGAACTGAAAGAAACC | Real-time PCR |
| 3′ihtB-805 | TGCCATTGTCCAGCTTGTC | ||
| fimA | fimA88F | CGGAACGAATAACCCAGAGA | Real-time PCR |
| fimA88R | CTGACCAACGAGAACCCACT | ||
| fimAProm-F | CGACGCTATATGCAAGACAA | Generating biotin-labeled fimA promoter region for EMSA | |
| fimAProm-R | Bio-TGTAACGGGTTCTGCCTCGT | ||
| mfa | mfa121F | CAGATGGGTTGTTGCTCA | Real-time PCR |
| mfa121R | ATAGAAAGTGCTGCTGGTAG |
a
The underlined sequences indicate either restriction enzyme sites or sequences corresponding to the sequences of ermF-ermAM gene.