TABLE 3.
Expression levels of hmuY mRNA in P. gingivali 33277 are not altered by the absence or presence of hemin when compared under similar cell density conditions
| Growth condition of 33277 | Growth period (h) | OD600 | mRNA levela:
|
||
|---|---|---|---|---|---|
| hmuY | pg1237 | pg1737 | |||
| With hemin | 24 | 1.76 ± 0.05 | 1.00 | 1.00 | 1.00 |
| 8 | 0.22 ± 0.01 | 166.57 ± 3.60 | 5.18 ± 0.11 | 0.80 ± 0.01 | |
| Without hemin | First 32 | 1.60 ± 0.04 | 1.17 ± 0.10 | 1.58 ± 0.03 | 1.62 ± 0.21 |
| Second 16 | 0.21 ± 0.01 | 172.45 ± 5.67 | 5.35 ± 0.10 | 0.95 ± 0.06 | |
a
Expression levels of hmuY, pg1237, and pg1737 mRNA were determined by using real-time RT-PCR. The mRNA levels of each gene in P. gingivalis 33277 grown under different conditions are indicated relative to the expression level in the presence of hemin for 24 h as 1 unit. The results shown are the means and standard deviations from three independent experiments.