Domains in the GALLS proteins. Boxes indicate the locations of the ATP binding sites (Walker A and B), helicase motif III, NLS, GALLS repeats 1 to 3, and type IV secretion signal. Restriction sites relevant to gene manipulation are shown. The GALLS gene encodes GALLS-FL and GALLS-CT, which results from translation initiated at an in-frame start codon (Met 808). The bottom boxes depict tryptic peptides of GALLS-CT detected by MS as indicated on the figure.