TABLE 1.
GFP diffusion coefficient versus growth osmolality
| Growth osmolality (osmol) | na | 〈φ〉b |
 (δDIQR)c (μm2·s−1) |
DGFP (μm2·s−1)
|
Growth rate (h−1)e | |
|---|---|---|---|---|---|---|
| Mean ± SDd | Range | |||||
| 0.10 | 54 | 0.15 | 12.3 (3.5) | 12.3 ± 2.8 | 4.2-18.4 | 0.43 ± 0.02 |
| 0.28 | 106 | 0.16 | 14.1 (4.4) | 13.8 ± 3.8 | 4.9-21.6 | 0.55 ± 0.02 |
| 0.65 | 24 | 0.19 | 13.2 (4.4) | 13.3 ± 3.2 | 8.0-19.5 | 0.37 ± 0.02 |
| 1.02 | 25 | 0.25 | 10.3 (4.4) | 9.9 ± 3.1 | 4.8-16.3 | 0.19 ± 0.01 |
| 1.10 | 48 | 0.26 | 8.5 (2.1) | 8.8 ± 2.1 | 4.9-14.5 | 0.12 ± 0.01 |
| 1.22 | 34 | 0.28 | 8.7 (2.1) | 8.9 ± 2.5 | 4.1-15.5 | |
| 1.45 | 24 | 0.36 | 6.0 (3.0)f | 6.2 ± 2.2 | 2.9-10.9 | 0.06 ± 0.01 |
a
Number of individual cells measured.
b
Determined as described in the supplemental material.
c
For definitions, see the text.
d
For single measurements.
e
Generations per hour ± 1 standard deviation.
f
We find two morphologies for cells grown in 1.45 osmol MBM (Fig. 1). One is thread-like (50% of cells), while the other is ellipsoidal (50%), with an aspect ratio similar to those of cells at other growth osmolalities.