TABLE 2.

Effects of plasmolysis on the GFP diffusion coefficient for cells grown in 0.28 osmol MBM and osmotically upshifted

Total osmola	〈φ〉b	nc	(δDIQR)d (μm2·s−1)	DGFP (μm2·s−1)
				Mean ± SDe	Range
0.28	0.16	106	14.1 (4.4)	13.8 ± 3.8	4.9-21.6
0.41	0.19	28	10.2 (4.3)	9.9 ± 3.0	3.9-16.0
0.61	0.25	21	5.0 (3.1)	5.0 ± 1.9	1.4-7.5
0.85	0.29	20	1.7 (1.7)	2.0 ± 1.1	0.53-4.9
1.02	0.30	34	0.82 (0.9)	1.1 ± 0.9	0.12-4.7
1.25	0.33	14	0.20 (0.22)	0.9 ± 1.8	0.04-5.5

^aTotal osmolality after plasmolysis with NaCl of cells resuspended from growth in minimal MBM at 0.28 osmol. Uncertainty is ±0.005 osmol due to variations in solution preparation; measurement uncertainty is ±0.003 osmol.

^bDetermined as described in the supplemental material.

^cNumber of individual cells measured.

^dFor definitions, see the text.

^eFor single measurements.