FIG. 4.
ssDNA-dependent ATPase activity of DnaBBA. The ATPase activity of DnaBBA was measured in the presence of ssDNA cofactors. Reactions were carried out in a standard ATPase assay (see Materials and Methods) with 25 ng of DnaBBA and the indicated amounts of ssDNA and/or oligonucleotides. (A) Plots of ATPase activities with ssDNA cofactors. The data were analyzed by nonlinear regression analysis using Prism 6.0 (GraphPad Software). (B) Vmax and Kd values with each oligonucleotide or ssDNA as determined from the ATPase plots.