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Southern blot analysis of the asaP1 gene. Chromosomal DNA from Aeromonas salmonicida subsp. achromogenes strain Keldur265-87 was digested with the indicated restriction enzymes and probed with a 1,043-bp fragment of the asaP1 gene. Lane 1, 1,043-bp asaP1 fragment used as a probe; lane 2, XmaI; lane 3, SfiI (the probe contains a SfiI restriction site); lane 4, BstXI; lane 5, BstBI.
