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PulD-mCherry multimers composed of full-length PulD-mCherry subunits support pullulanase secretion. Strains PAP9124 without or with pCHAP580 (PulS+++) (lanes 1 and 2 and lanes 5 and 6, respectively) and PAP9138 without or with pCHAP580 (lanes 3 and 4 and lanes 7 and 8, respectively) were grown in minimal medium in the presence of 0.4% maltose to induce all Pul factors in order to monitor secretion of pullulanase. For other details, see the legend to Fig. 1.
