FIG. 7.

In situ detection of PulD by PulD-specific affitin Sac70*40-GFP. After fixation and permeabilization, PulD was detected by fluorescence microscopy using the PulD-binding artificial protein Sac7*40-GFP as a fluorescent probe. The fluorescence image is shown in the right panels; the corresponding phase-contrast image is shown on the left. (A) Microscopy of cells expressing pulAB, pulC to -O, and pulS at chromosomal levels (PAP7232 with maltose). (B) Microscopy of an isogenic strain lacking pulD (PAP7447 with maltose). Bar, 5 μm.