TABLE 1.
Strains and plasmids
| Strain or plasmid | Characteristics | Reference |
|---|---|---|
| Strains | ||
| PAP105 | Δ(lac-pro) pAPIP501 [F′(lacIq ΔlacZM15 pro+ Tn10)] (Tcr) | Laboratory collection |
| MC4100 | F−araD139 ΔlacU169 relA1 rpsL150 thi mot flb-5301 deoC ptsF25 rbsR | Laboratory collection |
| NS30 | MC4100 degP | Laboratory collection |
| PAP7232 | MC4100 malP::(pulS pulAB pulC to -O) F′ (lacIqpro+ Tn10) (Tcr) | 25 |
| PAP7447 | MC4100 malP::(pulS pulAB pulC to -O ΔpulD) F′ (lacIqpro+ Tn10) (Tcr) | 15 |
| PAP9123 | PAP7447Δ(λattL-lom)::bla lacIqp204-mCherry | This study |
| PAP9124 | PAP7447Δ(λattL-lom)::bla lacIqp204-pulD | This study |
| PAP9138 | PAP7447Δ(λattL-lom)::bla lacIqp204-pulD-mCherry | This study |
| PAP9210 | PAP105Δ(λattL-lom)::bla lacIqp204-pulD-mCherry | This study |
| PAP9312 | NS30Δ(λattL-lom)::bla lacIqp204-pulD | This study |
| PAP9313 | NS30Δ(λattL-lom)::bla lacIqp204-pulD-mCherry | This study |
| Plasmids | ||
| pCHAP3671 | pUC18-pulD | 14 |
| pDSW204 | Modified ptrc promoter in pTrc99A | 37 |
| pCHAP580 | pSU19-pulS | 14 |
| pCHAP7539 | pCHAP3671 AvrII | This study |
| pCHAP7542 | pCHAP3671-pulD-mCherry | This study |
| pCHAP7545 | pDSW204-mCherry | This study |