Fig. 7.

RT-PCR analysis of candidate Kv channel isoform expression in coronary arterioles. A: RT-PCR experiments established that transcripts of Kv3.1 and Kv3.3 were expressed in coronary arterioles, whereas Kv1.1, Kv3.2, and Kv3.4 mRNA were not detected. B: quantitative real-time PCR analysis revealed that expression levels of Kv3.1 and Kv3.3 were not altered by the hypercholesterolemic diet as determined using the 2^−ΔΔCT method. Expression levels are reported relative to GAPDH in the same samples. C: RT-PCR amplification of all candidate isoforms with cDNA prepared from porcine brain mRNA as a positive control. PCR-amplified products were electrophoresed on a 1.5% agarose gel and visualized with ethidium bromide staining. Marker is 123-bp DNA ladder. D: amplification of GAPDH from Yucatan brain in the presence (+RT) and absence (−RT; negative control) of reverse transcriptase in the reaction mixture. A no-template control sample is also included using GAPDH primers. Marker is 50-bp DNA ladder. Values in B are means ± SE of the number of animals in parentheses. P ≤ 0.05.