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Heat shock response in ssd1Δ cells. Wild-type (WT), hsp104Δ, or ssd1Δ cells carrying either an empty vector (vec) or a plasmid carrying the SSD1-V or ssd1-d allele were examined for the expression of Hsp104 (A), Hsp26 (B), Ssa1 (C), and Ydj1 (D) by immunoblotting (IB) with appropriate antibodies. Cells were left untreated (U) or were heat shocked (T; 37°C for 30 min and 46°C for 30 min).
