TABLE 1.
Plasmids, yeast strains, and oligonucleotides used in this study
| Plasmid, strain, or oligonucleotide primer | Description, genotype, or sequence | Reference or source |
|---|---|---|
| Plasmid | ||
| p425cFFL-GFP | FFL fused to GFP | 44 |
| pPL092 | SSD1-v (JK9-3da allele) in pRS316 | 34 |
| pPL093 | SSD1-d (W303a allele) in pRS316 | 34 |
| pAG32 | HYG B resistance cassette | 50 |
| p2HGPD | Empty vector for p2HGPDHsp104 | S. Lindquist |
| p2HGPDHsp104 | Plasmid containing Hsp104 under the GPD promoter | S. Lindquist |
| pRS416GAL | Empty vector for pRS416GALHsp104 | S. Lindquist |
| pRS416GALHsp104 | Plasmid containing Hsp104 under the Gal promoter | S. Lindquist |
| S. cerevisiae strain | ||
| BY4741 | MATahsi3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Open Biosystems |
| orfΔ | MATahsi3Δ1 leu2Δ0 met15Δ0 ura3Δ0 orf::KanMX | Open Biosystems |
| Y8835 | MATα ura3Δ::nat R can1Δ::STE2pr-Sp_his5 lyp1Δ his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 LYS2+ | 45 |
| Y8835hygR | MATα ura3Δ::hygR can1Δ::STE2pr-Sp_his5 lyp1Δ his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 LYS2+ | This study |
| Primer | ||
| SSD1-A | 5′TGGTACCCTAAACATTTTGGTCTTA3′ | |
| SSD1-B | 5′CCCAGGATTATTGCTATTGTTATTG3′ | |
| SSD1-C | 5′AGTTCATGGAGATCAACTACCTTTG3′ | |
| SSD1-D | 5′TTGTAAATATTGAAAAGAAGGCTGC3′ | |
| SSD1-dF | 5′ATTAAACGTTGGCCAATCACATC3′ | |
| SSD1-dR | 5′TTTCCAATAAGGACAGGGTGG3′ | |
| SSD1-V | 5′GAATTTTACGGACACTAATGAGTAC3′ | |
| Ssd1-d | 5′GAATTTTACGGACACTAATGAGTAG3′ | |
| YPL101W-A | 5′GCGATAATCAAGGAGAATATCAGTG3′ | |
| YPL101W-D | 5′TCTGTCTATTTCAAATCCAAAGGAG3′ | |
| YEL013W-A | 5′AAATCTGAATAATTTCTCTTTCCCG3′ | |
| YEL013W-D | 5′TATACTACGGAACAAAGACAGCCTC3′ | |
| YCL036W-A | 5′GGTCCAGAGTAATCCTGATGTTCTA3′ | |
| YCL036W-D | 5′CGTTATATGAGATTAATGGCCAAAG3′ | |
| YCL056C-A | 5′TGTTTGAGCTCGATACTAACTTCCT3′ | |
| YCL056C-D | 5′GTAAAGACTTGCAAAACCTGTCAAT3′ | |
| YOL081W-A | 5′TGCCCAACGATTATCTATTCTACAT3′ | |
| YOL081W-D | 5′ACAGAAACACTTTCAACTAAGACGG3′ | |
| Ura3HygRFdel | 5′AGTTTTGACCATCAAAGAAGGTTAATGTGGCTGTGGTTTCAGGGTCCATACAGCTGAAGCTTCGTACGC3′ | |
| Ura3HygRRdel | 5′TCTTTCCAATTTTTTTTTTTTCGTCATTATAGAAATCATTACGACCGAGATTCCCGGGCATAGGCCACTAGTGGATCTG3′ | |
| YCL060C-A | 5′TTTAACGTACCTATCCATTCCGTTA3′ | |
| YCL060C-D | 5′TATCCTCTCCGATATTATCACCGTA3′ | |
| YGR240C-A | 5′TTCCGCTCTTAATAAAGGAGTTTTT3′ | |
| YGR240C-D | 5′GATTAAGCACACCCTAAAACTTGAA3′ | |
| YLR414C-A | 5′TATTTCCAAATCGGGCGTACTAT3′ | |
| YLR414C-D | 5′ATTATTTCTGGCTCTTCTCCATTTT3′ | |
| YCR009C-A | 5′ACTTTTAGGTTAGCGGAGAAGATGT3′ | |
| YCR009C-D | 5′CGTATCTTATTCCTCGCTTCTATTG3′ | |
| YIL040W-A | 5′GCCACAGACAGCTATCTCTATGAAT3′ | |
| YIL040W-D | 5′TTCTGGATGGAAAGATGGATAAGTA3′ | |
| YOR360C-A | 5′GCACAATTTTTCCTCTTTTCTTTTT3′ | |
| YOR360C-D | 5′TTTAGATTACACCTGTTTTTGCACC3′ | |
| YMR032W-A | 5′CCGAGGTATATGATTTCCTCTTTGGG3′ | |
| YMR032W-D | 5′AGTTACAAATCCGGAGAGGTGTCCTT3′ | |
| YBL094C-A | 5′GTTTGATCTAGACGGTACAGGAAAA3′ | |
| YBL094C-D | 5′GAAGATGCACCATGTCTTATTCAGT3′ | |
| YJL183W-A | 5′ATCCAGGAAGAGATTAACCAGCTAT3′ | |
| YJL183W-D | 5′AAAAGCTTCTTCTTCTTCTTCCTTG3′ |