FIG. 6.
Acquired responsiveness to PPARγ is dependent on PPARγ-S112 in Cdk7 knockdown MEFs. PPAR reporter (PPRE3-TK-Luc) activity in MEFs transfected as indicated with control (siControl) or Cdk7 (siCdk7) siRNAs and either PPARγ or P∼PPARγ-S112A, demonstrating that in contrast to wild-type PPARγ, the nonphosporylatable mutant displays similar activities in siCdk7 and siControl MEFs. Values represent averages from three technical replicates from two independent experiments, with standard deviations shown by error bars.