TABLE 1.

Effect of Hsp72 knockdown on p21 and ERK activation in tumor cell lines

Cell line	Tissue type	Oncogene product	p53 type	Result fora:
				p21 increase	ERK activation	Senescence
MCF7	Breast	PIK3CA	WT	+	−	+
BT20	Breast	PIK3CA	Mutant	−	−	−
HCT116	Colon	PIK3CA + K-RAS	WT	+	+	+
			KO	−	+	+
A549	Lung	K-RAS	WT	−	+	+
OVCAR5	Ovary	K-RAS	WTb	−	+	+
PANC1	Pancreas	K-RAS	Mutant	−/+	+	+
MDA-MB231	Breast	K-RAS + B-RAF	Mutant	−	+	+
Hs578T	Breast	K-RAS	Mutant	−	+	+

^a+, positive; −, negative.

^bAlthough according to information provided by NCI, the OVCAR5 line has normal p53 (22), we could detect neither p53 nor p21 under normal conditions or with Hsp72 depletion or UV irradiation (not shown).