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E2F1 is required for CUX1 activation of these promoters. Kit 225 cells (A) or Hct 116 cells (B) were cotransfected with the indicated reporter constructs together with empty vectors or vectors encoding p110 CUX1, E2F1, or a truncated DN-E2F1 protein. Results are expressed relative to the response of cells transfected with empty vectors and stimulated with IL-2 for 24 h. (C) Control blot for myc-CUX1 and HA-E2F1 expression in transfected cells.
