FIG. 7.

CUX1 recruitment on the MKLP1, Ect2, and RacGAP promoters during cell cycle progression. ChIP was performed using anti-CUX1 or control (Ctrl; anti-HA) antibodies, as described in Materials and Methods, on nuclear extracts prepared at various time points from IL-2-stimulated Kit 225 cells. Positions of the PCR-amplified regions for each promoter are shown above the promoter scheme; primers used are listed in File S3 in the supplemental material. Input represents 2% of the starting material. The data are representative of at least two independent ChIP experiments. IP, immunoprecipitation.