FIG. 8.

IL-2 is required for CUX1 to stimulate activity of the MKLP1, Ect2, and RacGAP promoters. (A) Kit 225 cells were cotransfected with the promoter constructs together with the empty pXJ vector or pXJ encoding p110 CUX1 and then cultured for an additional 12 or 24 h with or without IL-2. (B) Cells were treated as in panel A except that LY294002 (5 μM) and rapamycin (5 nM) (Ly/Rapa) were added to IL-2-stimulated cells to prevent cycle progression. Results are expressed as the percentage of the response obtained with IL-2 alone.