FIG. 9.

E2F1 recruitment on the MKLP1 (A), Ect2 (B), and RacGAP (C) promoters during cell cycle progression. ChIP was performed using anti-E2F1 or control (Ctrl; anti-HA) antibodies on nuclear extracts prepared at various time points from IL-2-stimulated Kit 225 cells. Positions of the PCR-amplified regions are shown above the promoter scheme, and primers used are listed in File S3 in the supplemental material. The data are representative of at least two independent ChIP experiments. IP, immunoprecipitation.