FIG. 3.

Abnormal development of cortex, hippocampus, and cerebellum in Dab1 phosphorylation site mutants. (A) Abnormal development of cortex and hippocampus. The arrows indicate the abnormal marginal zone (black arrows) and CA1 region of the hippocampus (white arrows). DG, dentate gyrus. (B) Partial development of cerebellum in dab1^ab and dab1^cd homozygotes. Compared with the WT and the dab1^5F/5F mutant, a partial phenotype was seen in dab1^ab and dab1^cd homozygotes. Sagittal sections were prepared from P20 brains and stained with Nissl stain. (C) Purkinje cell migration defects in dab1^ab and dab1^cd mice. Sagittal sections of P20 cerebellum were stained with anti-calbindin and 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI). Some Purkinje cells migrated properly, but many were misplaced in clusters below or in the granule layer (arrowheads). Scale bars, 500 μm (A), 1 mm (B), and 50 μm (C).