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. 2008 Nov 3;29(2):448–457. doi: 10.1128/MCB.01006-08

FIG. 6.

FIG. 6.

Cell death assay in C. elegans. (A) Transgenic crn-4(tm1415) animals expressing wild-type CRN-4, (B) CRN-4(D15A/E17A/D115A), or (C) CRN-4(1-206) under the control of the dpy-30 promoter were generated. For each construct, data were collected from three independent transgenic lines (smEx). The stages of transgenic embryos examined were: comma, 1.5-fold, 2-fold, 2.5-fold, 3-fold, and 4-fold. The y axis represents the average number of cell corpses scored, and error bars indicate standard deviations. Fifteen embryos were counted for each developmental stage. Statistical analyses were carried out by using the Student t test. The data of significant difference are indicated with asterisks: *, P < 0.0001; and **, P < 0.0005. There are no statistical differences between the crn-4 mutant and transgenic animals.