FIG. 5.
Liver regeneration is normal in Rad50-deficient cells, but hepatocytes exhibit indices of DNA damage. (A) Kidney and liver sections from control and Rad50Δ/ind Mx-Cre+ mice were immunohistochemically stained with Rad50 antibody. Examples from mice sacrificed 4 weeks after pI-pC injection and 6 days post-PH are shown. (B) Percent liver weight recovery was calculated, and results were graphed for control and mutant mice up to 8 days post-PH. (C) Immunohistochemical staining of liver sections prepared from control and mutant mice were used to determine the percent γ-H2AX-positive hepatocytes before and after PH. Examples of γ-H2AX-labeled sections from Rad50+/− and Rad50Δ/− livers at 2 days post-PH are shown. quiesc, quiescent.