FIG. 6.

Alterations in the transcriptome of the HGs: effects of age, sex, and Aoh2 deletion. The gene expression data obtained with the use of whole-genome microarrays were analyzed after stratification for sex, age, and Aoh2 deletion. (A) The PCA of the VSN intensity values (means centered) of the 3,468 genes that were significant for any of the factors (Aoh2^−/− versus WT, age/sex groups, or interaction) after two-way ANOVA in the eight experimental groups. Comp, component. (B) Genes that were most significantly affected by AOH2 deletion (P < 0.001 for the “knockout” (KO) factor after two-way ANOVA and ≥2-fold change in at least one of the four groups). Genes and samples were clustered using Euclidean distance and average linkage. According to PCA, young animals cluster together, and show similar changes, while minimal changes are apparent in mature animals. Symbols: ♂, male; ♀, female. Abbreviations: a, adult; y, young.