FIG. 5.

USP19 interacts with KPC1 and, like KPC1, is localized to the cytosol. (A) Interaction between endogenous KPC1 and USP19. FR3T3 cell lysates were subjected to IP using anti-KPC1 antibody, followed by immunoblot analysis with anti-USP19 or anti-KPC1 antibodies. As a control (CTL) for specificity of the antibodies, the experiment was also conducted in cells in which USP19 had been depleted by RNA interference. IgG, immunoglobulin G. (B) FR3T3 cells were transfected with the indicated combinations of plasmids expressing Flag-USP19 and HA-KPC1. Extracts of the transfected cells were subjected to IP using anti-Flag or anti-HA antibody. The immunoprecipitates were blotted with the indicated antibodies. +, present; −, absent. (C) Subcellular fractions of lysate from FR3T3 cells were subjected to immunoblotting with anti-USP19, anti-KPC1, anti-histone H2A (nuclear marker), and anti-α-tubulin (cytoplasmic marker) antibodies.