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. Author manuscript; available in PMC: 2009 Dec 5.
Published in final edited form as: Neurosci Lett. 2008 Sep 25;447(1):42–47. doi: 10.1016/j.neulet.2008.09.050

Figure 4.

Figure 4

Confirmation of antiserum specificity by Western blot analysis. Shown are detergent-solubilized (NP-40) protein fractions electrophoretically separated using 15% SDS PAGE. Two representative trial blocks are shown (horizontal bars) for sham treatment (S), contralateral control OB (O, open), and ipsilateral naris-occluded OB (C, closed). H, rat hippocampus. Top blot: proBDNF dimer is observed following a short exposure (30 s) of the film. Middle blot: Same blot, but a longer exposure (5 min) was used to visualize the pro-BDNF monomer (Monomer) and mature BDNF (Mature). Bottom blot: Nitrocellulose was stripped and reprobed for beta-actin to demonstrate equal protein loading across treatments.