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. Author manuscript; available in PMC: 2008 Dec 30.
Published in final edited form as: Nature. 2008 Jul 9;454(7206):899–902. doi: 10.1038/nature07117

Figure 2. The discontinuous hammerhead ribozyme-containing 3′ UTRs (hcu) self-cleave in vitro.

Figure 2

Reverse transcriptase primer extensions of in vitro transcribed mouse Clec2d (D-hcu, lane 5) and Clec2e (E-hcu, lane 7) 3′ UTRs show that both RNAs are cleaved at a single location (open arrowheads).D-hcu cleavage site 5′ of U1.1 was identified by dideoxy sequencing of transcription products. Uncleaved E-hcu and triple mutant DM1-hcu are indicated with a black arrowhead. Asterisks indicate non-extended labelled primers.