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. Author manuscript; available in PMC: 2008 Dec 30.
Published in final edited form as: J Biol Chem. 2005 Oct 5;280(49):40398–40401. doi: 10.1074/jbc.C500140200

FIGURE 4. Mitochondrial CREB is involved in the neuronal survival.

FIGURE 4

A, mito-A-CREB cells (panels e and f) were highly susceptible to 3-NP-induced cytotoxicity compared with mito-ECFP (panels a and b) and mito-wt-CREB cells (panels c and d). Cells were treated with vehicle (control) and 3-NP (0.5–5 mM) for 72 h. B, cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Data are the average ± S.E. of three separate experiments: significant at p < 0.05 (*) and p < 0.01 (**). C, cytochrome c release is higher in mito-A-CREB cells (lane 3) compared with mito-ECFP (lane 1) and mito-wt-CREB cells (lane 2) in response to 3-NP. Cytosol fractions were prepared from cells treated with vehicle (control) or 3-NP (0.5 mM) for 72 h.