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. 2008 Dec;125(4):570–590. doi: 10.1111/j.1365-2567.2008.02872.x

Figure 3.

Figure 3

Effect of B-cell-activating factor (BAFF) on B-cell receptor (BCR)-induced and CD20-induced apoptosis in MLMA cells. (a) MLMA cells were treated with either rabbit anti-μ heavy-chain polyclonal antibody (mAb) (αμ, 10 μg/ml, left panel) or a combination of anti-CD20 mAb (αCD20, 5 μg/ml) and secondary rabbit anti-mouse immunoglobulin antibody (RαM, 5 μg/ml) (right panel) for 48 hr in the presence or absence of different concentrations of BAFF as indicated and binding with fluorescein isothiocyanate (FITC)-conjugated annexin V was examined as in Fig. 2(a). (b) MLMA cells preincubated with or without 400 ng/ml of BAFF for the indicated periods were treated with either αμ (left panel) or a combination of αCD20 and RαM (right panel) and examined as in (a). (c) The effect of APRIL on apoptosis induction was also examined as in (a). (d) MLMA cells were treated as in (a) and apoptosis was induced. The inhibitory effect of simultaneous addition of BAFF (200 ng/ml) against apoptosis was examined at different time-points as in (a).