Figure 1.

WKYMVm induced superoxide release from murine neutrophils. Murine neutrophils (5 × 10⁵ cells) were isolated from bone marrow and incubated with chemiluminescence (CL) reagents, including isoluminol and horseradish peroxidase, at 37° for 2 hr either alone (control) or with pertussis toxin (PTX) (500 ng/ml final concentration), after which reactive oxygen species (ROS) production was triggered by the addition of WKYMVm (100 nm) or phorbol 12-myristate 13-acetate (PMA) (50 nm; inset). The extracellular superoxide release was measured using an isoluminol-amplified CL technique. Representative kinetics of the CL response from three experiments are shown. Abscissa, time of CL recording (min); ordinate, the CL response (arbitrary units).