Figure 3.
Effects of WRW4 and cyclosporine H on WKYMVm-induced superoxide anion production. Murine neutrophils (5 × 105 cells) were isolated from bone marrow and primed with tumour necrosis factor (TNF)-α at 37° for 30 min before stimulation with WKYMVm (5 nm). The indicated concentrations of the specific formyl peptide receptor (FPR) antagonist cyclosporine H (open boxes) or the FPRL1 antagonist WRW4 (closed boxes) were included in the 5-min pre-warming period before the addition of WKYMVm. Superoxide release upon WKYMVm stimulation was continuously recorded using the chemiluminescence (CL) technique, and the results are expressed as percentage inhibition compared with samples without any antagonist. The data presented are from one representative experiment of three.