Table 2.
The list of transcripts associated with cuticle hardening that are up-regulated in intermolt (Cy5) crabs when compared against crabs in the post-molt stage (Cy3).
|
Clone IDs (Accession #) |
BLAST results | Score (Bits) | E value | M * | t * | P-value* |
Adjusted P-value* |
| PpHEM7 (EF110537) |
AY861677 Cancer magister hemocyanin AAW57890 hemocyanin C. magister | 290 455 |
6e-75 1e-126 |
-5.147 -4.716 -4.432 |
-10.466 -17.112 -14.02 |
0 0 0 |
0.004 0.002 0.002 |
| PpHEM3 (EF110533) |
AF249297 Callinectes sapidus hemocyanin subunit mRNA AAW57891 hemocyanin subunit 3 C. magister |
807 432 |
0.0 1e-119 |
-4.849 -3.951 |
-16.719 -14.337 |
0 0 |
0.002 0.002 |
| PpTRY1 (EF120993) |
AB205408 Seriola quinqueradiata mRNA for trypsinogen CAA75310 trypsin Litopenaeus vannamei |
75.8 318 |
2e-10 2e-85 |
-4.655 -4.434 -4.193 -4.154 |
-9.19 -13.924 -11.15 -12.959 |
0 0 0 0 |
0.007 0.002 0.004 0.003 |
| PpHEM1 (EF110531) |
AY861676 C. magister hemocyanin subunit 1 AAW57889 hemocyanin subunit 1 C. magister |
103 335 |
9e-19 1e-90 |
-4.473 -4.393 -4.112 |
-9.907 -15.061 -15.684 |
0 0 0 |
0.005 0.002 0.002 |
| PpTRY2 (EF120994) |
AAL67442 trypsin Paralithodes camtschaticus | 62.0 | 1e-08 | -4.473 | -10.021 | 0 | 0.005 |
| PpHEM4 (EF110534) |
AF249297 C. sapidus hemocyanin subunit mRNA AAF64305 hemocyanin subunit C. sapidus |
870 472 |
0.0 6e-132 |
-4.402 -4.295 -4.027 -3.954 -3.784 -3.75 -3.707 -2.331 |
-14.753 -14.847 -15.206 -14.946 -14.715 -14.898 -12.568 -6.86 |
0 0 0 0 0 0 0 0.001 |
0.002 0.002 0.002 0.002 0.002 0.002 0.003 0.018 |
| PpHEM2 (EF110532) |
AY861677 C. magister hemocyanin subunit 2 AAW57890 hemocyanin subunit 2 C. magister |
176 160 |
8e-41 1e-47 |
-4.273 | -9.162 | 0 | 0.007 |
| PpHEM5 (EF110535) |
AF249297 C. sapidus hemocyanin subunit AAW57893 hemocyanin subunit 5 C. magister |
327 195 |
3e-86 2e-89 |
-4.18 | -12.698 | 0 | 0.003 |
| PpHEM6 (EF110536) |
AF249297 C. sapidus hemocyanin subunit mRNA AAW57891 hemocyanin subunit 3 C. magister |
807 432 |
0.0 1e-119 |
-4.155 -4.083 -3.999 -3.977 -3.946 -3.869 -3.518 |
-16.298 -11.88 -15.838 -14.718 -15.306 -14.029 -13.331 |
0 0 0 0 0 0 0 |
0.002 0.003 0.002 0.002 0.002 0.002 0.003 |
| PpTRY3 (EF120995) |
Y15041 PVTRYPSN3 Penaeus vannamei trypsin gene CAA75311 trypsin Litopenaeus vannamei |
63.9 253 |
6e-07 3e-66 |
-4.119 -4.017 -3.972 -3.246 -3.225 |
-10.005 -11.911 -12.269 -8.569 -10.838 |
0 0 0 0 0 |
0.005 0.003 0.003 0.008 0.004 |
| PpHEM8 (EF110538) |
AF249297 C. sapidus hemocyanin subunit AAF64305 hemocyanin subunit C. sapidus |
95.6 109 |
3e-16 3e-22 |
-3.41 | -11.878 | 0 | 0.003 |
| PpCTRY1 (EF120996) |
CAA71673 Chymotrypsin BII L. vannamei | 32.7 | 4.9 | -2.935 | -9.127 | 0 | 0.007 |
| EF120998 | AAD16454 clotting protein precursor Pacifastacus leniusculus (crayfish) | 38.9 | 0.10 | -2.83 | -8.196 | 0 | 0.01 |
| PpCTRY2 (EF120997) |
HAY12273 H. armigera (cotton bollworm – arthropod) mRNA for putative serine protease (chymotrypsin-like protease) CAA71672 Chymotrypsin BI Litopenaeus vannamei |
50.1 159 |
0.008 6e-38 |
-2.433 -2.021 |
-8.984 -6.564 |
0 0.001 |
0.007 0.021 |
| EF120999 | CAD20734 carcinin Carcinus maenas (antibacterial protein) | 71.2 | 2e-11 | -1.696 | -5.514 | 0.002 | 0.036 |
M = log2 fold change in expression, t = t-statistic, and the P value = t-distribution. The adjusted P values were calculated using the False Discovery Rate (FDR) procedure. Negative values of M and t indicate up-regulation in the Cy5 sample (* multiple values represent several cDNAs all forming one contig).