Table 4.
A list of transcripts associated with cuticle hardening that are up-regulated in late pre-molt (Cy3) crabs when compared against crabs in ecdysis (Cy5).
| Clone IDs | BLAST results | Score (Bits) | E value | M * | t * | P-value* | Adjusted P-value* |
| Carcinin (EF120999) |
CAD20734 carcinin Carcinus maenas (antibacterial protein) | 71.2 | 2e-11 | 4.769 4.52 4.366 4.358 4.32 4.209 4.176 |
13.448 14.309 17.315 10.443 16.541 12.818 15.864 |
0 0 0 0 0 0 0 |
0.01 0.01 0.01 0.015 0.01 0.01 0.01 |
| PpCTLR (EF120992) |
AAP58737 C-type lectin receptor Paralabidochromis chilotes | 46.6 | 8e-04 | 4.178 3.938 3.929 3.831 |
16.607 14.064 14.175 11.36 |
0 0 0 0 |
0.01 0.01 0.01 0.013 |
| PpHEM1 (EF110531) |
AY861676 Cancer magister hemocyanin subunit 1 AAW57889 hemocyanin subunit 1 C. magister |
103 335 |
9e-19 1e-90 |
3.953 3.942 3.842 |
12.659 10.385 10.802 |
0 0 0 |
0.01 0.015 0.015 |
| EF120998 | AAD16454 clotting protein precursor Pacifastacus leniusculus (crayfish) | 38.9 | 0.10 | 3.764 | 14.388 | 0 | 0.01 |
| PpHEM3 (EF110533) |
AF249297 Callinectes sapidus hemocyanin subunit mRNA AAW57891 hemocyanin subunit 3 C. magister |
807 432 |
0.0 1e-119 |
3.714 3.26 |
11.321 10.152 |
0 0 |
0.013 0.015 |
| PpHEM4 (EF110534) |
AF249297 C. sapidus hemocyanin subunit mRNA AAF64305 hemocyanin subunit C. sapidus |
870 472 |
0.0 6e-132 |
3.688 3.654 3.247 3.214 3.207 3.163 3.16 2.075 |
10.413 12.337 9.711 10.436 9.52 11.682 8.346 7.813 |
0 0 0 0 0 0 0.001 0.001 |
0.015 0.01 0.016 0.015 0.017 0.012 0.025 0.029 |
| PpHEM7 (EF110537) |
AY861677 C. magister hemocyanin AAW57890 hemocyanin C. magister | 290 455 |
6e-75 1e-126 |
3.628 3.548 3.187 |
8.628 10.607 7.652 |
0.001 0 0.001 |
0.022 0.015 0.03 |
| PpHEM6 (EF110536) |
AF249297 C. sapidus hemocyanin subunit mRNA AAW57891 hemocyanin subunit 3 C. magister |
807 432 |
0.0 1e-119 |
3.572 3.565 3.429 3.37 3.297 3.211 2.979 |
9.35 13.009 10.33 9.005 7.107 9.707 9.247 |
0 0 0 0 0.001 0 0 |
0.018 0.01 0.015 0.02 0.037 0.016 0.018 |
| PpTRY3 (EF120995) |
Y15041 PVTRYPSN3 Penaeus vannamei trypsin gene CAA75311 trypsin Litopenaeus vannamei |
63.9 253 |
6e-07 3e-66 |
3.474 3.278 3.024 2.892 |
8.198 12.823 6.46 6.616 |
0.001 0 0.002 0.002 |
0.025 0.01 0.048 0.047 |
| PpHEM5 (EF110535) |
AF249297 C. sapidus hemocyanin subunit AAW57893 hemocyanin subunit 5 C. magister |
327 195 |
3e-86 2e-89 |
3.339 | 12.09 | 0 | 0.01 |
| PpHEM2 (EF110532) |
AY861677 C. magister hemocyanin subunit2 AAW57890 hemocyanin subunit 2 C. magister | 176 160 |
8e-41 1e-47 |
3.33 | 10.225 | 0 | 0.015 |
| PpTRY1 (EF120993) |
AB205408 Seriola quinqueradiata mRNA for trypsinogen CAA75310 trypsin L. vannamei |
75.8 318 |
2e-10 2e-85 |
3.275 3.122 |
12.784 7.468 |
0 0.001 |
0.01 0.033 |
| PpTRY2 (EF120994) |
AAL67442 trypsin Paralithodes camtschaticus | 62.0 | 1e-08 | 2.769 | 6.565 | 0.002 | 0.047 |
M = log2 fold change in expression, t = t-statistic, and the P value = t-distribution. The adjusted P values were calculated using the False Discovery Rate (FDR) procedure. Positive values of M and t indicate up-regulation in the Cy3 sample (* multiple values represent several cDNAs all forming one contig).