Figure 3.

CRF injection increases KOR phosphorylation. A, Representative Western blots showing that CRF (1 μg/5 μl, i.c.v.) increased the 65 kDa KORp-ir band (lane 2) in striatal extracts compared with saline injection (lane 1). Pretreatment with norBNI (10 mg/kg, i.p.) blocked the CRF-induced increase (lane 3). The bar graph quantifies the increase in KORp-ir intensity and norBNI antagonism evident in replicate gels. *p < 0.05, significantly different from saline (n = 4–12 animals per group). B, Representative images showing a substantial increase in KORp-ir (green) in the basolateral amygdala (BLA) after injection of CRF compared with saline-injected mice. The increased KORp-ir was not evident in mice pretreated with norBNI (CRF + NorBNI) or in Pdyn(−/−) mice injected with CRF. GAD67-ir is also shown (red), and the bottom row of images is shown at higher power. Data represent three independent experiments taken from separate animals.