Fig. 3.

Effect of L. donovani infection on Pam3cys or LPS stimulated cytokine production by human monocytes. Isolated monocytes were infected with L. donovani at 10:1 parasites to cell ratio and stimulated with Pam3cys (10 ng/ml) or LPS (10 ng/ml). Supernatants were collected after 24 h of stimulation and IL-12p40 and IL-10 was measured by ELISA. Data represents mean + SD of five individuals. Group comparison was done using student's t test P < 0·05 considered significant (*P < 0·05, **P < 0·005, ***P < 0·0005).