Fig. 4.

Effect of L. donovani infection on Pam3cys and LPS stimulated phosphorylation of MAPK P³⁸ and ERK1/2. THP-1 cells were infected with L. donovani at 10:1 parasites to cell ratio and simultaneously stimulated with Pam3cys (1 µg/ml) (A, B) or LPS (1 µg/ml) (C, D) for 0, 5, 15 and 30 min. Cells lysates were subjected to electrophoresis, transferred on nitrocellulose membrane and probed with antibodies to phosphorylated and non-phosphorylated P³⁸ and ERK1/2. The results are expressed as ratio of densitometric readings of phosphorylated P³⁸ (A, C) and ERK1/2 (B, D) to non-phosphorylated P³⁸ and ERK1/2. Mean + SD of three independent experiments is shown.